Land, Air and Sea, DOT Drug Testing USA - Local Drug Testing Centers Nationwide | Land, Air and Sea, DOT Drug Testing USA - Local Drug Testing Centers Nationwide | Land, Air and Sea, DOT Drug Testing USA - Local Drug Testing Centers Nationwide

DOT Random Drug Testing

DOT Random Drug Testing

DOT Drug Testing USA provides a full range of DOT-required testing services that comply with 49 CFR Part 40 including DOT random drug testing, DOT breath alcohol testing, DOT consortium program, DOT supervisor training, and DOT drug policy development.

Drug testing centers are located nationwide and within minutes of your home or office and same-day service is available in most cases.

To schedule a DOT Random drug test or to join the DOT random pool/consortium, Schedule online 24/7 or Call (800)579-8083.

As an employee regulated by DOT, you are subject to unannounced random drug & alcohol testing. Alcohol testing is administered just prior to, during, or just after performing safety-sensitive functions. Depending on the industry-specific regulations, you may only be subject to random drug testing. USCG & PHMSA do not perform random alcohol tests. No manager, supervisor, official, or agent may select you for testing just because they want to. Under DOT regulations, employers must use a truly random selection process. Each employee must have an equal chance to be selected and tested. Just prior to the testing event, you will be notified of your selection and provided enough time to stop performing your safety-sensitive function and report to the testing location. Failure to show up for a test or interfering with the testing process can be considered a refusal to test.

All safety-sensitive employees must be a member of a DOT random selection pool/consortium in accordance with DOT random drug testing selection percentages.

The DOT random drug test is a 5-panel urine drug test that must be analyzed by a SAMHSA Certified Laboratory and verified by a Medical Review Officer. A DOT drug test screens for the following,

  • Amphetamines
  • Opiates
  • Cocaine
  • Phencyclidine (PCP)
  • Marijuana


DOT Agency
Federal Motor Carrier (FMCSA)50% Drugs10% Alcohol
Federal Aviation Administration (FAA)25% Drugs10% Alcohol
Federal Railroad Administration (FRA)25% Drugs10% Alcohol
Federal Railroad Administration (FRA) (MOW)
25% Drugs10% Alcohol
Federal Railroad Administration (FRA) (Mechanical)
50% Drugs25% Alcohol
Federal Transit Administration (FTA)50% Drugs10% Alcohol
Pipeline Hazardous Materials (PHMSA)25% DrugsN/A
United States Coast Guard (USCG)50% DrugsN/A


To review the Department of Transportation (DOT) drug testing regulations – CLICK HERE

Avoid DOT fines and penalties, be compliant with all DOT drug and alcohol testing regulations including DOT random drug testing requirements.

DOT Drug Testing USA can schedule your DOT random drug test, Call us today!

U.S. Department of Transportation

Online Order/Scheduling Registration

Please select your DOT mode and proceed to order selection. The zip code you enter will be used to locate the closest testing center to you.

Have Questions or Need Help, Call our DOT Scheduling Department at (800) 579-8083
(800) 579-8083